factors that affect the extraction operation

PH, temperature, salting out

PH effect

Effect of pH on apparent partition coefficient

PH low is favorable for the distribution of acidic substances in the organic phase, and alkaline substances are distributed in the aqueous phase

The weak acid decreases with the decrease of K

The weak base decreases with decreasing pH

The effect of temperature

* T increased, the molecular diffusion rate accelerated, so the extraction speed

* T affects the partition coefficient

 Substance-T Increased solubility in water increases

So T is reduced at the time of extraction to raise K

T affect the dissociation balance of weak acid and weak base

* T affects the mutual solubility of the two solvents

General extraction of biochemicals is carried out at room temperature or at a lower temperature

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